stat6 (tyr641) polyclonal antibody  (Bioss)

Journal: iScience

Article Title: Sleep deprivation-induced sympathetic activation promotes pro-tumoral macrophage phenotype via the ADRB2/KLF4 pathway to facilitate NSCLC metastasis

doi: 10.1016/j.isci.2025.112321

Figure Lengend Snippet: KLF4 is associated with sleep deprivation-induced ADRB2 modulation of macrophage prompting on proliferation, migration, and invasion of LLC-LUC cells (A) Differentially expressed Top20 genes in metastatic lung tissues of NC group and sleep deprivation group. (B) The expression of top 5 upregulated genes in tumor tissues was analyzed by qRT-PCR. (C) KLF4 expression was detected by ADRB2-specific agonist ISO and ADRB2 antagonist ICI118,551 in macrophages treated with IL-4 in vitro . (D) The effect of ADRB2 agonist on CD206 expression in IL-4-induced KLF4-knockdown macrophages. (E) EdU assays showed the proliferation of tumor cells in the coculture system with 30% CM. CM derived from IL-4 and IL-4 + ISO induced supernatant of macrophages with KLF4 low and high expression. (F) The migration ability of tumor cells was determined by wound healing assays in the indicated coculture medium. (G) Transwell assays demonstrated invasion of tumor cells in the indicated coculture system. (H) Representative IHC images of p-JAK1 and p-STAT6 in xenograft tumors. (I) The phosphorylation of JAK1 and STAT6 expression in RAW264.7 treated with ADRB2 agonist or antagonist via Western blotting. (J) Immunoblotting of KLF4 in RAW264.7 treated with different conditions. Data represented as mean ± SD. Statistical analysis was performed using Student’s t test or Mann-Whitney U test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: The paraffin sections of mice tumor tissues were subjected to baking, dewaxing, dehydration, elimination of endogenous peroxidase activity, tissue pressure-resistant antigen repair, 5% BSA blocking, dropping of rabbit polyclonal antibody against KLF4 (Proteintech), rabbit polyclonal antibody against CD31 (Proteintech), rabbit polyclonal antibody against p-JAK1 (ImmunoWay), rabbit polyclonal antibody against p-STAT6 (ImmunoWay), PBS washing at 4°C overnight, incubation with secondary antibody at 37°C for 35 min, PBS washing, and staining.

Techniques: Migration, Expressing, Quantitative RT-PCR, In Vitro, Knockdown, Derivative Assay, Phospho-proteomics, Western Blot, MANN-WHITNEY

Journal: iScience

Article Title: Sleep deprivation-induced sympathetic activation promotes pro-tumoral macrophage phenotype via the ADRB2/KLF4 pathway to facilitate NSCLC metastasis

doi: 10.1016/j.isci.2025.112321

Figure Lengend Snippet: NE level is elevated in NSCLC patients with sleep deprivation and is associated with a poor prognosis (A) The concentration of NE in serum of the NSCLC patients, determined by ELISA assay. (B) Representative IHC images and quantitative analysis of ADRB2 in the NSCLC patients. (C) Representative IHC images and quantitative analysis of KLF4 in the NSCLC patients. (D) Investigation into the association between ADRB2 expression and immune cell infiltration in patients diagnosed with LUAD. (E) Analysis of the correlation between KLF4 and immune cell infiltration in LUAD patients. (F–H) Correlation analysis between ADRB2, KLF4, JAK1, and STAT6 expression. (I and J) Correlation analysis between KLF4, JAK1, and STAT6 expression. (K and L) Correlation analysis between JAK1, STAT6, and pro-tumoral macrophages. Data represented as mean ± SD. Statistical analysis was evaluated using Student’s t test. ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: The paraffin sections of mice tumor tissues were subjected to baking, dewaxing, dehydration, elimination of endogenous peroxidase activity, tissue pressure-resistant antigen repair, 5% BSA blocking, dropping of rabbit polyclonal antibody against KLF4 (Proteintech), rabbit polyclonal antibody against CD31 (Proteintech), rabbit polyclonal antibody against p-JAK1 (ImmunoWay), rabbit polyclonal antibody against p-STAT6 (ImmunoWay), PBS washing at 4°C overnight, incubation with secondary antibody at 37°C for 35 min, PBS washing, and staining.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing

Journal: iScience

Article Title: Sleep deprivation-induced sympathetic activation promotes pro-tumoral macrophage phenotype via the ADRB2/KLF4 pathway to facilitate NSCLC metastasis

doi: 10.1016/j.isci.2025.112321

Figure Lengend Snippet:

Article Snippet: The paraffin sections of mice tumor tissues were subjected to baking, dewaxing, dehydration, elimination of endogenous peroxidase activity, tissue pressure-resistant antigen repair, 5% BSA blocking, dropping of rabbit polyclonal antibody against KLF4 (Proteintech), rabbit polyclonal antibody against CD31 (Proteintech), rabbit polyclonal antibody against p-JAK1 (ImmunoWay), rabbit polyclonal antibody against p-STAT6 (ImmunoWay), PBS washing at 4°C overnight, incubation with secondary antibody at 37°C for 35 min, PBS washing, and staining.

Techniques: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Phagocytosis Assay, Sequencing, Software

Journal: Cell reports

Article Title: The Janus kinase 1 is critical for pancreatic cancer initiation and progression

doi: 10.1016/j.celrep.2024.114202

Figure Lengend Snippet: (A) Western blot analyses of JAK1, JAK2, as well as tyrosine phosphorylated STAT proteins in human pancreatic cancer cell lines ( n = 9) and untransformed pancreatic cells (HPNE). Beta-actin (ACTB) and GAPDH served as loading controls; mouse pancreatic tumor cells treated with human growth hormone (hGH) and IL-4 served as controls for the activation of STAT5 and STAT6, respectively (b and c). Western blots are representative of three experimental replicates used for quantitative analysis in . (B) Quantitative analysis of nuclear STAT3 expression in primary human pancreatic cancers ( n = 28) and normal tissues ( n = 17). The right panel shows representative immunofluorescent (IF) images of normal and tumor tissues co-stained for tyrosine phosphorylated STAT3 (pSTAT3) and pan-cytokeratin (panCK). Slides were counterstained with DAPI; bars, 40 μm.

Article Snippet: Rabbit polyclonal, STAT6 , Santa Cruz , Cat#sc-981; RRID:AB_632450.

Techniques: Western Blot, Activation Assay, Expressing, Staining

Journal: Cell reports

Article Title: The Janus kinase 1 is critical for pancreatic cancer initiation and progression

doi: 10.1016/j.celrep.2024.114202

Figure Lengend Snippet: (A) Immunoblot analysis of STAT3 activation in mutant KRAS G12D /p53 R172H mouse pancreatic tumor cell lines before (—Cre) and after the conditional knockout of JAK1 or JAK2 (+Cre). (B) Western blot analysis of active STAT6 in two JAK1-deficient mouse PDAC cell lines and their JAK1-expressing parental controls with or without IL-4 administration. (C) Immunoblot analysis of STAT3 activation in two pairs of human PDAC cell lines (MIA PaCa-2, AsPC-1) before and after CRISPR-Cas9-mediated deletion of the JAK1 gene and the doxycycline (Dox)-inducible, short hairpin RNA (shRNA)-mediated knockdown of JAK2. ACTB and GAPDH were used as loading controls (A–C). (D) Comparative analysis of the relative numbers and sizes of tumorspheres in two mouse and one human isogenic cancer cell lines with and without JAK1. (E) Relative numbers of isogenic mouse and human pancreatic cancer cells that migrated after 24 h in a Transwell assay. Data are presented in panels D andE as the mean ± SD of three replicate experiments. Statistical significance was calculated with t tests; * p < 0.05 and ** p < 0.01. If not indicated otherwise, the differences were not significant.

Article Snippet: Rabbit polyclonal, STAT6 , Santa Cruz , Cat#sc-981; RRID:AB_632450.

Techniques: Western Blot, Activation Assay, Mutagenesis, Knock-Out, Expressing, CRISPR, shRNA, Knockdown, Transwell Assay

Journal: Cell reports

Article Title: The Janus kinase 1 is critical for pancreatic cancer initiation and progression

doi: 10.1016/j.celrep.2024.114202

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal, STAT6 , Santa Cruz , Cat#sc-981; RRID:AB_632450.

Techniques: Recombinant, Transduction, Polymer, Virus, Western Blot, Software

Journal: Journal of pharmacological sciences

Article Title: Seihaito, a Kampo medicine, attenuates IL-13-induced mucus production and goblet cell metaplasia.

doi: 10.1016/j.jphs.2024.02.008

Figure Lengend Snippet: Fig. 3. Effect of Seihaito on IL-13-induced STAT6 phosphorylation and SPDEF mRNA expression in ALI-cultured tracheal epithelial cells. ALI cultures of tracheal epithelial cells were treated with IL-13 (10 ng/ml) combined with Seihaito (1 mg/ml) or DEX (100 nM) for 7 days. Phosphorylation of STAT6 was assessed by western blotting (A), and SPDEF mRNA was measured by RT-real-time PCR (B). Data are presented as mean ± SE from three different experiments.

Article Snippet: Rabbit polyclonal anti-STAT6 antibody and rabbit monoclonal antiphospho-STAT6 antibody (Y641), mouse anti-β-actin antibody were purchased from Cell Signaling (MA, USA).

Techniques: Phospho-proteomics, Expressing, Cell Culture, Western Blot, Real-time Polymerase Chain Reaction

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Immunohistochemistry antibodies used in our study

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: a. Penile lesion, encapsulated and bossellated mass measuring 10x4.5x4 cm. b. hematoxylin and eosin (H&E) stain (100x) showing hypo and hypercellular areas with few staghorn-shaped vessels c. H&E stain (400x) demonstrates ovoid to spindle cells with homogenous-looking nuclei d. STAT6 immunohistochemistry (IHC) (100x) shows diffuse nuclear positive staining e. p53 IHC (100x) shows strong nuclear positive staining in nearly 70% of tumor cells f. BCOR IHC (100x) is negative.

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Staining, Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: a. Hematoxylin and eosin (H&E) (100x) showing spindle cells arranged haphazardly with a single hemangiopericytomatous (HPC)-like vessel, b. STAT6 immunohistochemistry (IHC)(100x) showing diffuse strong nuclear positive staining, c. Tumor cells are positive for CD34 IHC (100x) and d. BCL2 IHC (100x)

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry, Staining

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: a. Hematoxylin and eosin (H&E) (400x), Highly pleomorphic nuclei, with atypical mitotic figures highlighted by arrows b. STAT6 immunohistochemistry (IHC) (100x) diffuse nuclear positive c. BCOR IHC (100x) was negative (score 0) d. p53 IHC (100x) was negative (score 0).

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Results of immunohistochemistry analysis

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Differential diagnoses (DDs) considered in our study with regard to the cytoarchitecture

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Histopathology, Immunohistochemistry, Mutagenesis

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Other neoplasms that can also cause diagnostic dilemmas with solitary fibrous tumors

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Diagnostic Assay, Histopathology, Immunohistochemistry, Amplification, Mutagenesis, Variant Assay

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Immunohistochemistry antibodies used in our study

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: a. Penile lesion, encapsulated and bossellated mass measuring 10x4.5x4 cm. b. hematoxylin and eosin (H&E) stain (100x) showing hypo and hypercellular areas with few staghorn-shaped vessels c. H&E stain (400x) demonstrates ovoid to spindle cells with homogenous-looking nuclei d. STAT6 immunohistochemistry (IHC) (100x) shows diffuse nuclear positive staining e. p53 IHC (100x) shows strong nuclear positive staining in nearly 70% of tumor cells f. BCOR IHC (100x) is negative.

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Staining, Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: a. Hematoxylin and eosin (H&E) (100x) showing spindle cells arranged haphazardly with a single hemangiopericytomatous (HPC)-like vessel, b. STAT6 immunohistochemistry (IHC)(100x) showing diffuse strong nuclear positive staining, c. Tumor cells are positive for CD34 IHC (100x) and d. BCL2 IHC (100x)

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry, Staining

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: a. Hematoxylin and eosin (H&E) (400x), Highly pleomorphic nuclei, with atypical mitotic figures highlighted by arrows b. STAT6 immunohistochemistry (IHC) (100x) diffuse nuclear positive c. BCOR IHC (100x) was negative (score 0) d. p53 IHC (100x) was negative (score 0).

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Results of immunohistochemistry analysis

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Differential diagnoses (DDs) considered in our study with regard to the cytoarchitecture

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Histopathology, Immunohistochemistry, Mutagenesis

Journal: Cureus

Article Title: Exploring Solitary Fibrous Tumors at a Tertiary Cancer Center: Clinicopathological and Immunomorphologic Profile

doi: 10.7759/cureus.56899

Figure Lengend Snippet: Other neoplasms that can also cause diagnostic dilemmas with solitary fibrous tumors

Article Snippet: STAT6 rabbit polyclonal antibody, directed against the C terminus of STAT6 (EP325; Santa Cruz Biotechnology, Santa Cruz, California, USA), with dilution 1:50, was used (Table ).

Techniques: Diagnostic Assay, Histopathology, Immunohistochemistry, Amplification, Mutagenesis, Variant Assay

Journal: Frontiers in Immunology

Article Title: Major vault protein regulates tumor-associated macrophage polarization through interaction with signal transducer and activator of transcription 6

doi: 10.3389/fimmu.2023.1289795

Figure Lengend Snippet: MVP interacts with STAT6 and JAK1. (A) HA-tagged MVP and Flag-tagged STAT6 were co-transfected into HEK293T at 36 h post-transfection, and the cells were collected for Co-immunoprecipitation (Co-IP) and immunoblot analyses. (B) BMDMs were cultured in SF medium overnight after the formation of adherent cells. The next day, cells were stimulated with medium or IL-4 for 30 min and collected for Co-IP and immunoblot analyses. (C) HEK293T cells were transfected with pEGFP-MVP and DsRed-STAT6 for 36 h and then collected for IF assays. Representative image of MVP (green) and STAT6 (red), Scale bar, 10 µm (left panel). The quantitative analysis of co-localization using Image J (right panel). (D) Schematic diagram of structural domains and truncated constructs of STAT6 full-length (FL) (upper panel). HEK293T were co-transfected with HA-tagged MVP and truncated mutants of Flag-tagged STAT6 for 36 h. Then cells were collected for Co-IP and immunoblot analyses (lower panel). (E) Experiments were performed similarly to those in (D) , except the indicated truncated constructs of MVP were used. (F) Flag-JAK1 and HA-MVP were co-transfected into HEK293T for 36 h, and then the cells were harvested for Co-IP and immunoblot analyses. (G) BMDMs were stimulated with or without IL-4 for 30 min and collected for Co-IP and immunoblot analyses. (H) HEK293T cells were transfected with indicated plasmids for 36 h prior to Co-IP and immunoblot analyses. (I) Wt and Mvp -/- BMDMs were stimulated with medium or IL-4 for 30 min prior to Co-IP and immunoblot analyses. All protein abundance analyses were performed using Image J. All experiments were repeated at least three times with similar results. See also Supplementary Figure 7 .

Article Snippet: Antibodies for phospho-STAT6 (Tyr641) (CSB-PA000625) and phospho-STAT1 (Tyr641) (CSB-PA050162) were from Cusabio.

Techniques: Transfection, Immunoprecipitation, Co-Immunoprecipitation Assay, Western Blot, Cell Culture, Construct, Quantitative Proteomics

Journal: Frontiers in Immunology

Article Title: Major vault protein regulates tumor-associated macrophage polarization through interaction with signal transducer and activator of transcription 6

doi: 10.3389/fimmu.2023.1289795

Figure Lengend Snippet: MVP enhances the phosphorylation and nuclear translocation of STAT6. (A) Wt and Mvp -/- BMDMs were stimulated with or without IL-4 at the indicated times before western blot analysis. (B) Raw264.7 cells were transfected with Flag-tagged MVP or vector for 36 h, then stimulated with phosphate-buffered saline (PBS) or IL-4 for 30 min. Immunoblot analyses were performed with the indicated antibodies. (C) Wt and Mvp -/- BMDMs were stimulated with IL-4 for the indicated time. The whole cell lysates (WCL), cytosolic and nuclear extracts were prepared and subjected to western blot analyses. Lamin B and GAPDH were used as nuclear and cytosolic fractions markers, respectively. (D) Experiments were performed similar to those in (C) , except Raw264.7 cells were transfected with Flag-MVP for 36 h. (E) IF of STAT6 in Wt and Mvp -/- PMs stimulated with IL-4. Representative image of STAT6 (green). Scale bar, 20 µm (left panel). The percentage of nuclear STAT6 positive cell numbers was counted (right panel). All protein abundance analyses were performed using Image J. All experiments were repeated at least three times with similar results. Data are expressed as means ± SEM, n = 3, two-tailed Student’s t-test. (**P < 0.01). See also Supplementary Figure 8 .

Article Snippet: Antibodies for phospho-STAT6 (Tyr641) (CSB-PA000625) and phospho-STAT1 (Tyr641) (CSB-PA050162) were from Cusabio.

Techniques: Phospho-proteomics, Translocation Assay, Western Blot, Transfection, Plasmid Preparation, Saline, Quantitative Proteomics, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Major vault protein regulates tumor-associated macrophage polarization through interaction with signal transducer and activator of transcription 6

doi: 10.3389/fimmu.2023.1289795

Figure Lengend Snippet: MVP-promoted M2-TAMs polarization and tumorigenesis in HCC. In the tumor microenvironment of hepatocellular carcinoma, JAK1 recruits MVP and STAT6, leading to ternary complex formation. Then, STAT6 is phosphorylated and translocated from the cytosol to the nucleus. As a result, STAT6 binds to the promoter of M2 genes, leading to M2 polarization and M2-TAMs infiltration.

Article Snippet: Antibodies for phospho-STAT6 (Tyr641) (CSB-PA000625) and phospho-STAT1 (Tyr641) (CSB-PA050162) were from Cusabio.

Techniques: